Journal: ACS applied materials & interfaces

Article Title: Extracellular Vesicles Enhance the Remodeling of Cell-Free Silk Vascular Scaffolds in Rat Aortae.

doi: 10.1021/acsami.0c06609

Figure Lengend Snippet: Figure 2: A: Proliferation and B: migration of smooth muscle cells (SMCs) and endothelial cells (ECs) when exposed to extracellular vesicle (EV) based treatments. BM: Basal media; PBS: Phosphate buffered saline; EV50: 50 μl of EV isolate; EV150: 150 μl of EV isolate; SBM: Supplemented basal media. * represents p < 0.05, ** represents p < 0.005 and *** represents p < 0.0001.

Article Snippet: 22 Human coronary artery endothelial cells (ECs) were obtained from Cell Applications 23 (#300-05a), cultured in supplemented basal media (#212K-500, Cell Applications 24 Inc, SBM) and used at passage 4.

Techniques: Migration, Saline

Journal: Molecular Medicine Reports

Article Title: X-irradiation induces acute and early term inflammatory responses in atherosclerosis-prone ApoE − / − mice and in endothelial cells

doi: 10.3892/mmr.2021.12038

Figure Lengend Snippet: Inflammatory response in endothelial cells at 24 h after irradiation. The response of various inflammatory markers at 24 h after 0.1 and 5 Gy irradiation is represented in (A-I) TICAE cells and (J-R) TIME cells, normalized to cell count. The Kruskal-Wallis test was used to analyse the data and the P-value was adjusted using the Benjamini-Hochberg method. Values represent the average ± SEM of 6 biological replicates. *P<0.05, **P<0.01 and ***P<0.001 vs. 0 Gy. p.i., post irradiation; GDF-15, growth differentiation factor-15; CXCL10, C-X-C motif chemokine ligand 10; ICAM-1, intercellular adhesion molecule-1; MCP-1, monocyte chemoattractant protein-1; uPAR, urokinase-type plasminogen activator receptor; PAI-1, plasminogen activator inhibitor-1; FGF-basic, basic fibroblast growth factor.

Article Snippet: In addition, TICAE cells, which are primary human coronary artery endothelial cells from the European Collection of Authenticated Cell Cultures (ECACC; HCAECs cat. no. 300-05a) that were transduced with retroviruses bearing the est2 gene, a yeast homologue of the human TERT protein ( , ), were used.

Techniques: Irradiation, Cell Counting

Journal: Molecular Medicine Reports

Article Title: X-irradiation induces acute and early term inflammatory responses in atherosclerosis-prone ApoE − / − mice and in endothelial cells

doi: 10.3892/mmr.2021.12038

Figure Lengend Snippet: Inflammatory response in endothelial cells at 72 h after irradiation. The response of various inflammatory markers at 72 h after 0.1 and 5 Gy irradiation is presented in (A-I) TICAE cells and (J-R) TIME cells. Data were normalized to cell count. The Kruskal-Wallis test was used to analyse the data and the P-value was adjusted using the Benjamini-Hochberg method. Values represent the average ± SEM of 6 biological replicates. *P<0.05, **P<0.01 and ***P<0.001 vs. 0 Gy. p.i., post irradiation; GDF-15, growth differentiation factor-15; CXCL10, C-X-C motif chemokine ligand 10; ICAM-1, intercellular adhesion molecule-1; MCP-1, monocyte chemoattractant protein-1; uPAR, urokinase-type plasminogen activator receptor; PAI-1, plasminogen activator inhibitor-1; FGF-basic, basic fibroblast growth factor.

Article Snippet: In addition, TICAE cells, which are primary human coronary artery endothelial cells from the European Collection of Authenticated Cell Cultures (ECACC; HCAECs cat. no. 300-05a) that were transduced with retroviruses bearing the est2 gene, a yeast homologue of the human TERT protein ( , ), were used.

Techniques: Irradiation, Cell Counting

Journal: Diabetes & Vascular Disease Research

Article Title: Mitochondrial mitophagy protection combining rivaroxaban and aspirin in high glucose-exposed human coronary artery endothelial cell. An in vitro study

doi: 10.1177/14791641221129877

Figure Lengend Snippet: Panel a.- Representative Western-blot of the Factor Xa (FXA) expression in human coronary artery endothelial cells (HCAEC) incubated with normal D-glucose concentration (5mmol/L, control) and in the presence of 30 mmol/L D-Glucose (+Glucose). Panel b.- Representative Western-blot of tissue factor (TF) expression in HCAEC incubated with normal D-glucose concentration (5 mmol/L, control) or with high glucose, 30 mmol/L D-Glucose (+Glucose). Experiments adding 50 nmol/L Rivaroxaban, 0.33 mmol/L ASA and 12.5 nmol/L Rivaroxaban +0.33 mmol/L ASA to high D-glucose-incubated HCAEC are also represented. Panel c.- Representative dot-blots to measure mitochondrial content of Pink-1 and Parkin proteins HCAEC incubated with 5 mmol/L D- (control) or 30 mmol/L D-Glucose (+Glucose). Experiments adding 50 nmol/L Rivaroxaban, 0.33 mmol/L ASA and 12.5 nmol/L Rivaroxaban +0.33 mmol/L ASA to high D-glucose-incubated HCAEC are also represented. Panel d.- Reactive oxygen species (ROS) production and changes in mitochondria membrane potential (ΔΨm) in HCAEC incubated with 5 mmol/L D- (control) or 30 mmol/L D-Glucose (+Glucose). Experiments adding 50 nmol/L Rivaroxaban, 0.33 mmol/L ASA and 12.5 nmol/L Rivaroxaban +0.33 mmol/L ASA to high D-glucose-incubated HCAEC are also represented. All experiments were also performed in presence of submaximal thrombin concentration (0.025 U/mL). Bar graphs of the four panels show the densitometric analysis represented in arbitrary units (A.U.). Results are represented as mean ± SEM of six different experiments. * p < 0.05 compared to the experiments performed normal glucose (5 mmol/L, control). # p < 0.05 compared to the experiments performed with 30 mmol/L glucose (+Glucose).

Article Snippet: The human coronary artery endothelial cell (HCAEC) line, (Ref. 350–05a, Merck KGaA, Germany) was incubated under the following experimental conditions: HCAEC incubated with physiologic D-glucose concentration (5 mmol/L, control group), HCAEC incubated with 30 mmol/L D-Glucose to mimic an hyperglycemic condition (+Glucose group), HCAEC incubated with 30 mmol/L D-Glucose+50 nmol/L Rivaroxaban (Bay 59–7939, Rivaroxaban group), HCAEC incubated with 30 mmol/L D-Glucose+0.33 mmol/L acetylsalicylic acid (ASA group) and 30 mmol/L D-glucose incubated HCAEC with Rivaroxaban (12.5 nmol/L) +ASA (0.33 mmol/L) (Riva+ASA group).

Techniques: Western Blot, Expressing, Incubation, Concentration Assay

Journal: Metabolomics

Article Title: Metabolic fingerprints of human primary endothelial and fibroblast cells

doi: 10.1007/s11306-016-1024-7

Figure Lengend Snippet: Different phenotypes of human primary endothelial and fibroblast cells. Micrographs show normal, healthy endothelial cells isolated from coronary arteries, umbilical vein and fibroblasts from the lungs. HCAEC human coronary artery endothelial cells, HUVEC human umbilical vein endothelial cells and NHLF normal human lung fibroblasts

Article Snippet: HCAEC human coronary artery endothelial cells, HUVEC human umbilical vein endothelial cells and NHLF normal human lung fibroblasts Fig. 2 Optimization of Biolog media, redox dye mixes and cell density, for HCAEC human coronary artery endothelial cells and NHLF normal human lung fibroblasts.

Techniques: Isolation

Journal: Metabolomics

Article Title: Metabolic fingerprints of human primary endothelial and fibroblast cells

doi: 10.1007/s11306-016-1024-7

Figure Lengend Snippet: Optimization of Biolog media, redox dye mixes and cell density, for HCAEC human coronary artery endothelial cells and NHLF normal human lung fibroblasts. The OmniLog reading results, as area under curve , of the separate wells, are presented in green

Article Snippet: HCAEC human coronary artery endothelial cells, HUVEC human umbilical vein endothelial cells and NHLF normal human lung fibroblasts Fig. 2 Optimization of Biolog media, redox dye mixes and cell density, for HCAEC human coronary artery endothelial cells and NHLF normal human lung fibroblasts.

Techniques:

Journal: Metabolomics

Article Title: Metabolic fingerprints of human primary endothelial and fibroblast cells

doi: 10.1007/s11306-016-1024-7

Figure Lengend Snippet: HCAEC, HUVEC and NHLF assayed in PM-M1 to PM-M4. The columns show the endpoint of a 24 h incubation of a representative replicate of three independent experiments performed. Positive wells with glucose utilization are indicated with black boxes , while negative, background wells are shown with blue boxes . Exclusive results obtained for HCAEC are shown in green boxes and for NHLF in yellow boxes . HCAEC human coronary artery endothelial cells, HUVEC human umbilical vein endothelial cells and NHLF normal human lung fibroblasts

Article Snippet: HCAEC human coronary artery endothelial cells, HUVEC human umbilical vein endothelial cells and NHLF normal human lung fibroblasts Fig. 2 Optimization of Biolog media, redox dye mixes and cell density, for HCAEC human coronary artery endothelial cells and NHLF normal human lung fibroblasts.

Techniques: Incubation

Journal: Metabolomics

Article Title: Metabolic fingerprints of human primary endothelial and fibroblast cells

doi: 10.1007/s11306-016-1024-7

Figure Lengend Snippet: Comparison of substrate utilization in all three cell types are shown on plates PM-M1 and PM-M2, PM-M3 and PM-M4. Cells were assayed according to the standard protocol and data collected after 24 h using the OmniLog and PM software, with subtraction of the background. Average height (mOD) of tetrazolium reduction was measured in triplicate. HCAEC human coronary artery endothelial cells, HUVEC human umbilical vein endothelial cells and NHLF normal human lung fibroblasts

Article Snippet: HCAEC human coronary artery endothelial cells, HUVEC human umbilical vein endothelial cells and NHLF normal human lung fibroblasts Fig. 2 Optimization of Biolog media, redox dye mixes and cell density, for HCAEC human coronary artery endothelial cells and NHLF normal human lung fibroblasts.

Techniques: Comparison, Software

Journal: Metabolomics

Article Title: Metabolic fingerprints of human primary endothelial and fibroblast cells

doi: 10.1007/s11306-016-1024-7

Figure Lengend Snippet: Unique substrates metabolized exclusively by HCAEC, HUVEC and NHLF, as well as overlapping substrates, are represented by the Venn diagram. HCAEC human coronary artery endothelial cells, HUVEC human umbilical vein endothelial cells and NHLF normal human lung fibroblasts

Article Snippet: HCAEC human coronary artery endothelial cells, HUVEC human umbilical vein endothelial cells and NHLF normal human lung fibroblasts Fig. 2 Optimization of Biolog media, redox dye mixes and cell density, for HCAEC human coronary artery endothelial cells and NHLF normal human lung fibroblasts.

Techniques: